By Vedpal S. Malik, Erik P. Lillehoj
The applicability of immunotechniques to a large choice of analysis difficulties in lots of components of biology and chemistry has elevated dramatically over the past 20 years ever because the advent of monoclonal antibodies and complicated immunosorbent recommendations. Exquisitely particular antibody molecules offer technique of separation, quantitative and qualitative research, and localization priceless to a person doing organic or biochemical research.
This functional consultant to immunotechniques is principally designed to be simply understood by way of individuals with little useful event utilizing antibodies. It essentially offers precise, easy-to-follow, step by step equipment for the commonly used strategies that make the most the original homes of antibodies and may support researchers use antibodies to their greatest virtue.
* distinctive, easy-to-follow, step by step protocols
* handy, easy-to-use format
* huge sensible information
* crucial history information
* invaluable tricks
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Additional resources for Antibody Techniques
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Cell 73, 7 3 - 8 6 . Laver, W. , Air, G. , Webster, R. , and Smith-Gill, S. J. (1990). Epitopes on protein antigens: Misconceptions and realities. Cell 61, 5 5 3 - 5 5 6 . Linsley, P. , Grosmaire, L. , Damle, N. , and Ledbetter, ). A. (1991a). Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin-2 mRNA accumulation. /. Exp. Med. 173, 7 2 1 - 7 3 0 . Linsley, P. , Grosmaire, L. , Damle, N. , and Ledbetter, J. A. (1991b). CTLA-4 is a second receptor for the B cell activation antigen B7.
E. (1987). Interferon--)/ and B cell stimulatory factor-1 reciprocally regulate Ig isotype production. Science 236, 944-947. Snapper, C. , Finkelman, F. , Conrad, D. , and Paul, W. E. (1988). IL-4 induces co-expression of intrinsic membrane IgGl and IgE by murine B cells stimulated with lipopolysaccharide. /. Immunol. 141, 4 8 9 - 4 9 8 . Sprent, J. (1994). T and B memory cells. Cell 76, 3 1 5 - 3 2 2 . Stavnezer, J . , Lin, Y . - C , Nietupski, J . , and Severinson, E. (1988). Immunoglobulin heavy-chain switching may be directed by prior induction of transcripts from constant-region genes.
Antibody Techniques by Vedpal S. Malik, Erik P. Lillehoj