By Jennie P. Mather and David Barnes (Eds.)
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Extra info for Animal Cell Culture Methods
These are quite different in many ways and can have very different effects on the properties of cells grown in them. Additionally, serum varies from animal to animal, with changes in diet, and seasonally. Considerable variability therefore exists from lot to lot of commercially available sera. To make whole sera, the blood is allowed to clot and the clot removed, or the blood can be collected with an anticlotting agent and the cellular portion spun out, resulting in plasma. Serum and plasma, even from the same animal, are quite different in composition and in their effect on cells.
Make up medium using a different lot of medium powder, 2. Choosing the Appropriate Medium and Culture System 27 serum, and so on (or open another lot of tissue culture dishes) and test by comparing the newly made medium to the presumptive “bad” lot of medium. It is best to change only one thing at a time. 8. , a still in another laboratory) and test medium made with this water. Although all of these precautions may seem excessive, good quality control can save days and weeks spent tracking down problems that affect experimental outcome and can make the difference between success and failure in growing some types of cells.
1976). Many of these trace elements are normally provided as trace contaminants that enter the medium in water or serum. As the medium becomes more defined and the water more pure, these trace elements need to be purposely added to the medium formulation. Sometimes an increased concentration of vitamins can be useful. Vitamin mixtures are commercially available and can be added as such. Some vitamins, such as vitamin E (or a-tocopherol), A (retinol or retinoic acid), or C , are not added to media mixtures because of their instability, but may be important for some cells to survive or function in vitro.
Animal Cell Culture Methods by Jennie P. Mather and David Barnes (Eds.)