By Roland Wagner, Hansjörg Hauser
This e-book introduces the elemental ideas and sensible program of ideas utilized in the scalable creation of proteins and virus-based gene-therapeutic items with animal telephone cultures. The e-book stories a vast spectrum of topics proper to biologics construction, together with the construction of telephone strains, a survey of sensible genomics for mobilephone traces and strategies, and advances in upstream and downstream strategy improvement. it's a vital reference for all these operating within the biotechnology box either from a tutorial and commercial viewpoint.
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Importantly, the cells that went through crisis display a grossly altered phenotype when the established cell line is compared to their primary counterparts which is also reflected by an altered karyotype . Therefore, an efficient immortalization of primary human cells is only achieved with a concerted action of TAg with a second immortalizing gene such as c-myc or human telomerase (hTert, see below). 2 Immortalization by hTert and the Impact of Telomeres A critical part during genome duplication during S phase is the replication of the very ends of chromosomes; the repetitive DNA stretches which are called telomeres.
Hepatitis C virus core protein promotes immortalization of primary human hepatocytes. Virology 2000;271:197-204.  Kwakkenbos MJ, Diehl SA, Yasuda E, et al. Generation of stable monoclonal antibody-producing B cell receptor-positive human memory B cells by genetic programming. Nature Medicine 2010;16:123-8.  Horton SJ, Jaques J, Woolthuis C, et al. MLL-AF9-mediated immortalization of human hematopoietic cells along different lineages changes during ontogeny. Leukemia 2013;27:1116-26.  Klochendler A, Weinberg-Corem N, Moran M, et al.
84] Rybkin II, Markham DW, Yan Z, et al. Conditional expression of SV40 T-antigen in mouse cardiomyocytes facilitates an inducible switch from proliferation to differentiation. Journal of Biological Chemistry 2003;278:15927-34.  Zhang Y, Nuglozeh E, Toure F, et al.
Animal Cell Biotechnology by Roland Wagner, Hansjörg Hauser