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By Feng Zhang, Holger Puchta, James G. Thomson

ISBN-10: 1493925555

ISBN-13: 9781493925551

ISBN-10: 1493925563

ISBN-13: 9781493925568

Over the earlier 50 years, biotechnology has been the foremost motive force for expanding crop productiveness. fairly, advances in plant genetic engineering applied sciences have spread out gigantic new possibilities for plant researchers and breeders to create new crop types with fascinating qualities. contemporary improvement of detailed genome amendment equipment, corresponding to specific gene knock-out/knock-in and special gene alternative, strikes genetic engineering to a different point and provides much more potentials for making improvements to crop creation. The paintings offers an summary of the newest advances on unique genomic engineering applied sciences in crops. subject matters comprise recombinase and engineered nucleases-mediated special amendment, negative/positive selection-based homologous recombination and oligo nucleotide-mediated recombination. ultimately, demanding situations and affects of the hot applied sciences on current rules for genetic amendment organisms (GMOs) should be discussed.

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As indicated in Fig. 2, I-CreI works as a homodimer on a pseudo-palindromic target. The DNA–protein interaction is accomplished via a region involving 40 amino acids. As previously reported, each monomer can be divided into sub-domains. For instance, we described two sub-domains referred to as distal (10NNN) and proximal (5NNN) to the catalytic center (Fig. 2). These subdomains interact with the bases in positions ±8, ±9, ±10 and ±3, ±4, ±5 within the 2 Engineering Meganuclease for Precise Plant Genome Modification 25 Fig.

2014). Because all the meganucleases fused in megaTALs are monomeric endonucleases, the new hybrid nuclease does not require dimerization to cleave target sites. Moreover, DNA binding specificity of megaTALs is determined by both the TALE and meganuclease, reducing the likelihood that more than one target site would be present in the targeted genome. Thus, the fusion nucleases could offer higher specificity than regular meganucleases. In a recent research study, Boissel et al. have compared cleavage activity between megaTALs and their derived meganucleases, I-AniI and I-OnuI (Boissel et al.

Biochemistry 38(39):12621–12628 Ichiyanagi K, Ishino Y, Ariyoshi M, Komori K, Morikawa K (2000) Crystal structure of an archaeal intein-encoded homing endonuclease PI-PfuI. J Mol Biol 300(4):889–901 Jacquier A, Dujon B (1985) An intron-encoded protein is active in a gene conversion process that spreads an intron into a mitochondrial gene. Cell 41(2):383–394 Jarjour J, West-Foyle H, Certo MT et al (2009) High-resolution profiling of homing endonuclease binding and catalytic specificity using yeast surface display.

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Advances in New Technology for Targeted Modification of Plant Genomes by Feng Zhang, Holger Puchta, James G. Thomson


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